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The Alliance for Pathogen Surveillance Innovations (APSI)-India is a multi-city consortium of research, clinical, public and private institutions. Since its inception in 2021, the consortium has worked towards setting up advanced surveillance platforms to detect pathogens of public health concern, utilizing genome sequencing, wastewater-based detection techniques incorporating bioinformatics and data analytics tools. These platforms have been used to detect pathogens both in clinical and environmental samples. Wastewater based epidemiology is an approach to environmental disease surveillance that provides early warning signs for infectious disease agents via cost-effective measures of health. To conduct wastewater surveillance, APSI has established over 150 sampling sites across India (seen in map below), where sampling is conducted at regular intervals. The figure below depicts the number of sites and samples collected at specific locations. As part of conducting genomic surveillance during the second wave of COVID in 2021, APSI sequenced over 48,000 clinical samples across cities to report the then circulating SARS-CoV-2 variants (depicted in GISAID submissions table). You can read more about our vision and go through some of the stories from our efforts here.

GISAID submissions
Hyderabad
Bengaluru
Pune
Total
8314
20195
21573
50082

Number of SARS-CoV-2 genome sequences uploaded to GISAID

Delhi
Samples
Collected
715
Wastewater
Locations
20
Pune
Samples
Collected
5495
Wastewater
Locations
42
Hyderabad
Samples
Collected
3028
Wastewater
Locations
49
Bengaluru
Samples
Collected
5276
Wastewater
Locations
28

Scientific Session on APSI's role in environmental surveillance at MICROCON 2024

Pune
Nov, 30 2024

Bangalore: 2nd Hands-on workshop for molecular approaches, pathogens and AMR surveillance

Bengaluru
Nov, 18 2024

Bangalore-Science and Community: One Health series

Bengaluru
Nov, 12 2024

Fostering Awareness: AMR Frontline Workshops in Tumkur and Bangalore ahead of World Antimicrobial Awareness Week

Bengaluru
Nov, 06 2024

Sustaining the Momentum: AMR Frontline Workshop at SIMSRC on Oct 14 – Bangalore

Bengaluru
Oct, 14 2024

AMR Frontline Workshop at BMCRI: A Successful Start to Our Series in Karnataka

Bengaluru
Sep, 30 2024

The Fourth All-Cluster Rockefeller Foundation-Alliance for Pathogen Surveillance Innovations (RF-APSI)-India Consortium Meet

Pune
Sep, 27 2024

Bangalore, Dengue Warrior’s Education and Awareness Program Series

Bengaluru
Sep, 17 2024

Bangalore: Hands-on workshop on Molecular diagnostic approaches for pathogens and AMR surveillance

Bengaluru
Sep, 16 2024

Bangalore, Outreach Event: RF-APSI Pathogen surveillance, outreach and way forward

Bengaluru
Sep, 13 2024

Hands on DNA Sequencing Workshop

Delhi
Aug, 29 2024

Antimicrobial research conference, August 22-23, 2024, TIGS|NCBS, Bangalore

Bengaluru
Aug, 22 2024

Bangalore NCBS Comms and RF APSI play Manju What’s up with you.

Bengaluru
Aug, 04 2024

“Molecular Approaches towards pathogen and AMR detection in wastewater Workshop” - CCMB, Hyderabad

Hyderabad
Jul, 29 2024

AMR Surveillance strategies and clinical approaches, CME workshop.

Bengaluru
Jun, 30 2024

SAKSHAM workshop at Gadhinglaj Panchayat Samiti, where 220 Multipurpose Workers (MPWs) attended from Kagal, Bhudargad, Aajara, Chandgad, Radhanagari and Gadhinglaj.

Pune
Jun, 21 2024

SAKSHAM workshop at Kolhapur Zilla Parishad Office and Hall, where 250 Multipurpose Workers (MPWs) attended from Shahuwadi, Hatkanangale, Shirol, Panhala, Gaganbawda and Karveer.

Pune
Jun, 20 2024

PKC-SaS workshops at BJ Medical College and Bharati Vidyapeeth Medical College, Pune

Pune
Jun, 09 2024

Rockefeller Foundation-Alliance for Pathogen Surveillance Innovations (RF-APSI)-India 3rd All Cluster Meet

Bengaluru
May, 23 2024

Shedding Light on the Invisible Threat: Ashoka University Workshop on Wastewater Surveillance of Antimicrobial Resistance

Delhi
Apr, 10 2024

Online AMR Frontline Workshop for Medical Students

Virtual Meeting
Mar, 02 2024

The second annual Rockefeller Foundation-Alliance for Pathogen Surveillance Innovations (RF-APSI)-India Consortium Meet

Hyderabad
Feb, 10 2024

AMR Frontline Workshop for Future Medics at GIMS

Delhi
Dec, 13 2023

AMR Frontline Workshops

Delhi
Dec, 12 2023

Pune Wastewater Surveillance Dashboard For Infectious Diseases (COVID-19, H1N1, H3N2, Influenza-A)

pune
Dec, 07 2023

SAKSHAM - Series of Workshops for Health Workers

pune
Dec, 07 2023

Focussed WWS discussion at the European Commission's International Conference ‘Towards a global waste surveillance system for public health’

Bengaluru
Nov, 17 2023

RF-PKC stakeholder meeting

pune
Aug, 02 2023

Focus group stakeholder meeting for- Infectious diseases

pune
Jul, 18 2023

Magnetize your Creativity 2.0

Hyderabad
Jul, 13 2023

One Health, Disease Surveillance and Pandemic Preparedness( G20-Chief Scientific Advisors Roundtable (CSAR) side event )

pune
Jul, 10 2023

Pen meets Petri Dish

Hyderabad
Jul, 01 2023

AMR Frontline Workshop

Hyderabad
Jun, 22 2023

Magnetize your Creativity

Hyderabad
May, 27 2023

Brushes against Superbugs

Hyderabad
Apr, 24 2023

Inter-Cluster Rockefeller Foundation (RF)-India Consortium Meet

Hyderabad
Mar, 04 2023

One Health City Bengaluru Consortium

Bengaluru
Mar, 03 2023

Design: Samruddhi Walaskar

Design: Samruddhi Walaskar

Design: Samruddhi Walaskar

Design: Samruddhi Walaskar

Design: Samruddhi Walaskar

Monitoring Influenza A (H1N1, H3N2), RSV, and SARS-CoV-2 Using Wastewater-Based Epidemiology: A 2-Year Longitudinal Study in an Indian Megacity Covering Omicron and Post-Omicron Phases
Rinka Pramanik, Kiran Nannaware, Vinita Malik, Priyanki Shah, Poornima Sangewar, Niharika Gogate, L. S. Shashidhara, Radhika Boargaonkar, Dhawal Patil, Saurabh Kale, Asim Bhalerao, Nidhi Jain, Sanjay Kamble, Syed Dastager & Mahesh Dharne
19/11/2024
Article
Wastewater surveillance in post-omicron silent phase uncovers silent waves and cryptic transmission of SARS-CoV-2 variants; a yearlong study in Western India
Vinay Rajput a b 1 , Rinka Pramanik a b 1 , Kiran Nannaware a , Vinita Malik a , Sejal Matra a , Shubham Kumar a , Sai Joshi a , Pradnya Kadam c , Unnati Bhalerao c , Manisha Tupekar c , Dipti Deshpande c , Priyanki Shah d , Poornima Sangewar d , Niharika Gogate d , Radhika Boargaonkar e , Dhawal Patil e , Saurabh Kale e , Asim Bhalerao f , Nidhi Jain f , L.S. Shashidhara c d g … Mahesh Dharne a b
11/10/2024
Article
Wastewater surveillance of severe acute respiratory syndrome coronavirus-2 in open drains of two Indian megacities captures evolutionary lineage transitions: a zonation approach
Saee Zambre, Poonam Katarmal, Shubhankar Pawar, Snehal Dawkhar, Parvati Iyer, Vinay Rajput, Pradnya Kadam, Unnati Bhalerao, Manisha Tupekar, Priyanki Shah, Krishanpal Karmodiya, Mahesh Dharne, Bishnudeo Roy & Santosh Koraktar
30/07/2024
Article
One Health Index Calculator for India: Using Empirical Methods for Policy Stewardship
Saveetha Meganathan, Arpit Katiyar, Esha Srivastava, Rakesh K Mishra
26/07/2024
Article
Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance
Jyotsna Karan1, Sreya Mandal1, Gulafsha Khan1, Harshit Arya1, Laasya Samhita1 1Ashoka University
19/07/2024
Article
Early detection of KP.2 SARS-CoV-2 variant using wastewater-based genomic surveillance in Pune, Maharashtra, India
Vinay Rajput, BTech, Rashmita Das, MD, Rinka Pramanik, MSc, Kiran Nannaware, MSc, Sushma Yanamandra, MD, Nyabom Taji, MD, Vishal Rajput, MSc, Riyakshi Rajkhowa, MSc, Poonam Pacharne, BSc, DMLT, Priyanki Shah, MSc, Niharika Gogate, MSc, Poornima Sangwar, MSc, Asim Bhalerao, MS, Nidhi Jain, MS, Sanjay Kamble, PhD, Syed Dastager, PhD, L S Shashidhara, PhD, Rajesh Karyakarte, MD, Mahesh Dharne, PhD
17/07/2024
Article
Reduced protein-coding transcript diversity in severe dengue emphasises the role of alternative splicing
Priyanka Mehta1,2, Chinky Shiu Chen Liu1, Sristi Sinha1 , Ramakant Mohite1 , Smriti Arora1 , Partha Chattopadhyay1,2, Sandeep Budhiraja3 , Bansidhar Tarai3 , Rajesh Pandey1,2
03/06/2024
Article
Antimicrobial resistance landscape in a metropolitan city context using open drain wastewater-based metagenomic analysis
Manas Kumar Madhukar , Nirupama Singh V Rajesh Iyer, Divya Tej Sowpati Karthik Bharadwaj Tallapaka , Rakesh Kumar Mishra, Shivranjani Chandrashekhar Moharir.
08/04/2024
Article
Metagenomic analysis reveals differential effects of sewage treatment on the microbiome and antibiotic resistome in Bengaluru, India
Annamalai Nataraj, Divya Mondhe, Vishwananth Srikantaiah, Farah Ishtiaq
17/01/2024
Article
Wastewater-based surveillance of vector-borne pathogens: a cautionary note
Farah Ishtiaq
29/12/2023
Article
Genomic sequencing should extend to diverse priority pathogens for effective study and surveillance of antimicrobial resistance: a systematic review of whole-genome sequencing studies from India
Nazneen Gheewalla, Jaisri Jagannadham, Rintu Kutum, Shraddha Karve
23/11/2023
Article
106_Brief Func Genomics_Bench to bedside translational research in COVID-19.pdf
Nityendra Shukla, Uzma Shamim, Preeti Agarwal, Rajesh Pandey and Jitendra Narayan
22/11/2023
Article
Genomic surveillance reveals early detection and transition of delta to omicron lineages of SARS-CoV-2 variants in wastewater treatment plants of Pune, India. Environ Sci Pollut Res (2023)
Rajput, V., Pramanik, R., Malik, V. et al
03/11/2023
Article
Defining a research agenda for environmental wastewater surveillance of pathogens
Alexander G. Shaw, Catherine Troman, Joyce Odeke Akello, Kathleen M. O’Reilly, Jillian Gauld, Stephanie Grow, Nicholas Grassly, Duncan Steele, David Blazes, Supriya Kumar & The Environmental Surveillance Working Group
01/09/2023
Article
A tale of two waves: Delineating diverse genomic and transmission landscapes driving the COVID-19 pandemic in Pune, India
Divya Niveditha, Soumen Khan, Ajinkya Khilari, Sanica Nadkarni, Unnati Bhalerao, Pradnya Kadam, Ritu Yadav, Jugal B. Kanekar, Nikita Shah, Bhagyashree Likhitkar, Rutuja Sawant, Shikha Thakur, Manisha Tupekar, Dhriti Nagarc, Anjani G. Rao, Rutuja Jagtap, Shraddha Jogi, Madhuri Belekar, Maitreyee Pathak, Priyanki Shah, Krishanpal Karmodiya
01/08/2023
Article
Ignoring wastewater is a wasted opportunity to improve disease response
Farah Ishtiaq
15/06/2023
Article
SARS-CoV-2 infection dynamics and genomic surveillance to detect variants in wastewater – a longitudinal study in Bengaluru, India
Sanjay Lamba, Sutharsan Ganesan, Namrta Daroch, Kiran Paul, Soumya Gopal Joshi, Darshan Sreenivas, Annamalai Nataraj, Vishwanath Srikantaiah, Rakesh Mishra, Uma Ramakrishnan, Farah Ishtiaq
01/04/2023
Article
Genomic surveillance reveals circulation of multiple variants and lineages of SARS-CoV-2 during COVID-19 pandemic in Indian city of Bengaluru.
Darshan Sreenivas, Vinay Kumar, Kandasamy Kathirvel, Rakesh Netha Vadnala, Sachin Mishra, Bhagyashree Shelar, Srilatha Marate, Lakshminarayanan CP, Sai Disha K, Manisha Bhardwaj, Awadhesh Pandit, Satyajit Mayor, Uma Ramakrishnan, Dimple Notani.
14/03/2023
Article
Campus Sewage Water Surveillance based dynamics and infection trends of SARS-CoV-2 variants during third wave of COVID-19 in Pune, India
Vinita Malik, Vinay Rajput, Rinka Pramanik, Rachel Samson, Rakesh kumar Yadav, Pradnya Kadam, Nikita Shah, Rutuja Sawant, Unnati Bhalerao, Manisha Tupekar, Soumen Khan, Priyanki Shah, LS Shashidhara, Sanjay Kamble, Syed Dastager, Krishanpal Karmodiya, Mahesh Dharne
06/03/2023
Article
India must scale up wastewater analysis for health surveillance
15/12/2022
Article
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Our Vision

Environmental surveillance has gained enormous significance in light of the COVID-19 pandemic and APSI-India envisions it as the way forward for monitoring infectious pathogens and preventing future pandemics. We believe that comprehensive monitoring of pathogens in strategic city locations is a key step in disease control and elimination strategies. For this purpose, we have developed, validated and aim to deploy highly advanced indigenized technologies to detect pathogens of public health concern at affordable costs. Our ultimate goal is to generate high-quality, data-driven surveillance tools that can be readily handed over to relevant agencies for large-scale implementation, contributing to robust and proactive public health systems in the country. Click here to view our detailed Protocols for surveillance of pathogens. APSI’s key objectives are highlighted below alongside our network chart.

APSI | our vision
Hyderabad
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Bengaluru
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Delhi
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Pune
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