Collection of wastewater samples from open drains

Processing of wastewater samples following biosafety measures in the laboratory

Hyderabad wastewater and clinical surveillance team

The CCMB, Hyderabad and AIIMS, Bibinagar RF-APSI team visiting open drains wastewater collection sites in Bibinagar, Telangana- May 2023.

Wastewater-based surveillance-the way ahead” workshop at CCMB on August 1st, 2023.

Medical students in the AMR Frontline Workshop at LVPEI

Winners of the Jamboree Quiz at the AMR Frontline workshop

Medical students looking at microbiology culture plates used for antibiotic susceptibility testing during the lab tours in the AMR Frontline Workshop at LVPEI

Medical students listening to Prof. Umabala Pamidimukulla (NIMS, Hyderabad) shedding light on infection control practices to curb AMR spread.

Dr. Ranga Reddy Burri (President, IFCAI) talking about antibiotics in animal farming and agriculture.

The Superheroes against Superbugs Team setting the stage for the AMR Frontline Workshop.

Wastewater site visit

Collection of samples following biosafety measures

Hands-on training in a workshop on use of bioinformatic tools for wastewater surveillance

Nucleic acid extraction from wastewater samples

Quantitative analysis of pathogens in wastewater

Transfer of sewage-treated water samples into containers.

Transfer of collected wastewater samples into sample containers after measuring 200ml of each sample.

After pasteurizing, sample processing is carried out inside the biosafety cabinet according to sample transfer and processing protocols.

Pasteurizing wastewater samples for sample inactivation using a hot bead bath at 60 degrees for 1 and half hours.

Sample collected from inlet of an STP site in Bangalore

Sample collection and processing.

Sewage treatment plant in Bangalore.

Dr. Spurgeon R., Director, Bangalore Baptist Hospital welcoming the audience, at launch of the June 30 AMR CME workshop, AMR-Surveillance strategies and clinical approaches.

Dr. Sufia Sadaf, Program Manager, Outreach, RF-APSI, NCBS-TIFR, Bengaluru speaking about the RF-APSI program and the relevance of dialog for broader outreach.

Dr. Rakesh Mishra, Director, TIGS and Principal Investigator-RF-APSI speaking about the RF-APSI program and its relevance for Public Health surveillance and actionable policy.

Dr. Shivranjani C Moharir, Senior Scientist, TIGS, speaking about the Environmental surveillance for pathogens and AMR landscape

Dr. Farah Ishtiaq, Principal Scientist, TIGS, speaking about wastewater and public health program policy surveillance in Bangalore city

Dr. Mansi Malik, Research Scientist, TIGS, speaking about AMR Clinical Surveillance using molecular diagnostics

Dr. Carolin E George, Head, Community Health & Research, Bangalore Baptist Hospital speaking about the relevance of joint dialog and CME program

Dr. Ansar Ahmad, State Surveillance Officer and Project Director, Interdisciplinary Surveillance Program, Directorate Health and Family Welfare, Govt. of Karnataka speaking about State surveillance AMR program under NCDC vision

Clinical Panel discussion on approaches to usage of antibiotic resistance in clinical fields led by Dr. Prasanna Kumar, Dr. Sudha Jasmine, Dr. Emmanuel Lazarus from CMC. Vellore and Dr. Indira Menon and Dr. Srividhya Raghavendran, from Bangalore Baptist Hospital

Diagnostic panel discussion covering antibiotic susceptibility tests, guidelines and stewardship programs led by Dr. Swati Rajagopal, Aster CMI, Dr. Abi Manesh, CMC Vellore and Dr. Sindhulina Chandrasingh, Bangalore Baptist Hospital

Case discussions based on real-life case studies ranging from medicine, family medicine, surgery, obstetrics & gynecology and critical care led by clinical panelists and clinical moderators

Panel discussion on joint platforms for public health surveillance to tackle AMR led by Dr. Anuj Sharma, National Professional Officer and Team Focal Point for AMR and IPC at WHO Country Office for India, Dr. Rakesh Mishra, Dr. Shivranjani C Moharir, Dr.Mansi Malik from TIGS, Dr. Bhaskar Rajakumar, ARTPARK, Dr. Carolin E George from Bangalore Baptist Hospital and moderated by Dr. Sufia Sadaf, RF-APSI, NCBS-TIFR, Bangalore.

Participants at the AMR workshop

Photo-booth by Superheroes against superbugs

Dr. Sufia Sadaf set the context of the welcome Dengue Warriors Education and awareness series, Bangalore for schools and teachers

Special Commissioner, Health and Animal Husbandry, BBMP Shri Suralkar Vikas Kishore inaugurating the program

Health Officer Dr. Savitha, BBMP addressing the audiencePhoto-booth by Superheroes against superbugs

Dr. Neha Mishra covering the dengue epidemiology and its medical relevance

Program series and ground partners for the Dengue warrior’s series

Mr. Ravi Tennety, Head of operations, Bengaluru Science and Technology, BeST cluster, an initiative of the office of PSA to the Gov.t of India, addressing the audience about utilization of science and technology to mitigate Dengue

Ms. Udbhavi Balakrishnan, Health reporter, Deccan Herald covering the importance of regulated media coverage for health reporting and spreading awareness

Dr. Bhaskar Rajakumar, Program Director, Health Initiatives, ARTPARK interacting with the audience about role of AI and data integration and dashboards for disease hotspots and predictions

Attendees of the first workshop held at NCBS

Infographic for mosquito life cycle to understand vectors for diseases

Closed-room interactions followed by outdoor exhibits by TIGS insectary team, ARTPARK and NCBS Comms team

Dengue-Warriors-education-series-Event-2-at-Bangalore-Creative-Circus

pledge-for-Dengue-warriors-and-ambassadors

Expert lectures, hands on exhbits to understand mosquitoes as vectors and their its life cycle and group interactions with school students

3rd Dengue warriors education series covering 9 schools and teachers

Jyoti Mehra, Dr. Sufia Sadaf, Disha Sai setting up the context for the Dengue warriors education series, covering the disease surveillance and epidemiology

Dr. Aditya Parekh, the Echo Network explaining the chatbot for dengue breeding hotspots and community participation. Shri P K Biswal, Education officer, VITM addressing the audience with vote of thanks

Interactive exhibits from ARTPARK, TIGS insectary team and VITM team including AI models, mosquito vector life cycle, virus models and laserbeam demonstration. An interactive kiosk added to the enthusiastic fervor

Hands on exhbits for pathogen-carrying vectors

Modules covered disease pathogens and surveillance strategies with infographics, films and visual narratives

Over 300 school students and community members attended the outreach event during the NCBS Open Day Sept 13

Quiz and questionnares became very popular

RF-APSI and TIGS joint Outreach for RF APSI pathogen surveillance

SciArt and quiz in progress

This event added to the ongoing outreach workshops at Bangalore APSI cluster

Volunteers explaining the APSI strategies for pathogen surveillance and outreach

Play on zoonosis and disease spread Manju whats up with you

Actors depicting the state of chaos due to fear of unknown

Shadow imagery to depict pathogens

zoonosis

platform depicting joint dialog amongst community members

Dengue warriors social media campaign participants and partners honored at the Sept 17 felicitation ceremony

School teacher and students honored for their efforts in the ongoing Dengue warriors campaign

Antimicrobial resistance research conference 2024 was held at NCBS campus

Opening talk by Dr Kamini Walia

Panel discussion on AMR surveillance

Poster session during ARC 2024

Welcome notes, expert lectures, interactive sessions and demos for hands on workshop

Hands on experiments and participation for sample processing, setting up PCR for wastewater samples and clinical samples followed by visits to NGS and BSL2 facilities

RT PCR being setup by the participants

Felicitation on the final day of the program

Sept 30, 2024 AMR workshop for medical college students at Bangalore Medical College and Research Institute, BMCRI, Bangalore led by APSI’s outreach partner SaS team

Poster launch during the inaugural session

Keynote address ‘Science of AMR and surveillance’ by Prof. LS Shashidhara, Director, NCBS, Bangalore,

Talk on ‘AMR stewardship and policies’ by Dr. Sangeetha S, Prof, Microbiology, RajaRajeshwari Medical College and Hospital

Talk on ‘Infection Control practices and AMR clinical challenges’ by Dr. Anitha Kommalur, Asst Prof, Dept of Pediatrics, BMCRI

Talk on ‘Novel therapeutics’ by Dr. Vasan Sambandamurthy, Sr Vice President, Global Strategy, Bugworks

Interactive sessions and educational games

Oct 14, 2024, AMR workshop for medical students at Saptagiri Instituite of Medical Sciences and Research, SIMSRC, Bangalore led by APSI’s outreach partner SaS team

Keynote address, ‘Introduction to AMR and molecular approaches for AMR surveillance’ by Dr. Mansi Malik, Research Scientist, TIGS, Bangalore

Talk on ‘AMR in clinical settings’ Dr. Kayva S, Associate Prof, Dept of Pediatrics, SIMSRC, Bangalore

Talk on ‘AMR policies and mitigation strategies’ Dr. Kavitha Karur, Asst Prof., Sri Jayadeva Institute of Cardiovascular Sciences and Research, Bangalore

Interactive sessions and educational games

Speakers addressing the Vijayawada municipal corporation personnel during the 2 nd hands-on workshop for molecular approaches for pathogens and AMR, organized by TIGS, NCBS, CCMB and CSIR-IICT, Nov 18-19, 2024

Hands-on experiments for pathogen surveillance, from wastewater for pathogen load analysis via qPCR

Learning and educational games to reinforce the workshop modules

Group picture for the 2 nd hands-training workshop, Nov 18-19, 2024 in Bangalore

Science and Community: One Health series prelaunch and pilot program, Nov 12, 2024 with NCBS, TIGS and VITM

Program launch, Science and Community: One Health series, Dec 20, 2024, with NCBS, TIGS, APSI and VITM as partners, expert talks by Prof. LS Shashidhara, Director, NCBS, Dr. Rakesh Mishra, Director, TIGS, Shri Sajoo Bhaskaran, Director, VITM, Prof. J Srinivasan, Emeritus Professor, IISc and Dr. Mansi Malik, Scientist, TIGS. The series will continue until Feb, 2025.

Panel discussion, interfacing the community with scientists, clinicians, animal health experts for the One health series, moderated by Dr. Sufia Sadaf, RF-APSI, NCBS and Ms. Jyoti Mehra, VITM, with panelists Dr. Rakesh Mishra, Dr. J Srinivsan, Shri Sajoo Bhaskaran, Dr Shivasharanappa Nayakvadi (Senior Scientist, ICAR-NIVEDI), Dr. Sindhulina Chandrasingh (Head – Laboratory Services & Consultant Microbiologist, Bangalore Baptist Hospital), Dr. Mansi Malik, and Dr. Shivranjani Moharir, TIGS.

Exhibits, activities and educational games for Public health surveillance and One Health, launched as part of ongoing exhibit, Vaccines-Injecting Hope at VITM.

Science and Community one health series, 3rd program

Science and Community one health series, 4th program

Science and Community one health program, SISF 2025, Puducherry

Inaugural Samartha tarabeti-1st cohort ASHA workers training and capacity building

Public health surveillance and AMR education for researchers and non-medical college students

Samartha tarabeti-2nd cohort ASHA training merged with 5th program for Science and Community One health series

Science and Community one health series, 6th program

Science and Community one health series, 7th program and finale

Bangalores 3rd hands-on training workshop_molecular surveillance for health personnel

GLC4HSR Annual Conclave, "Collaborative Learning to Coordinated Action: Policy and Practice for Resilient Health Systems."

Samartha tarabeti-3rd cohort ASHA training

Samartha tarabeti-4th cohort ASHA training

TIGS-ICMR IMPACT 2025, 1st cohort VRDL personnel training

Collection of wastewater samples from a nala

Pre-processing of wastewater samples

RT-PCR of wastewater samples in process

Speakers at Baramati Workshop on 25 Nov 2023: Dr. Suresh Dhekale (DMO Pune), Dr. Mangesh Gokhale (ex-NIV), Dr. Harshal Pandve from YCM, and Dr. Sunil Jagtap (Taluka Health Officer, Baramati)

Lecture session in progress at Ambegaon Workshop on 24 Jan 2024

Activity based learning session at Mawal Workshop on 21 Nov 2023

Participant engrossed in the activity at Wagholi workshop on 26 Oct 2023

Activity based learning session in progress at Baramati Workshop on 25 Nov 2023.

Impromptu feedback from the participants appreciating the workshop and thanking the organisers. Bhor Workshop on 23 Nov 2023.

Group photo after Workshop at Wagholi on 26 Oct 2023

Overview of PKC initiatives undertaken under Health Vertical, Ms Poornima Sangewar

Students attending the talk by an expert

Student explaining a group activity

Group activity being done by students

Dr Shraddha Karve, Associate Professor, Ashoka University, talking about AMR Surveillance

H.O.D (Microbiology) Dr Karyakartes address to the students at BJ Medical College, Pune

Group Activity

Dr Aarti Kinikar, HOD Pediatrics BJMC, address to the students at BJ Medical College, Pune

Mr Sachin Dube, CEO, Module Innovations Pvt. Ltd., addressing students about developing Diagnostic Tools for AMR

Group Activity - fast-paced AMR quiz

Drain no:6, Khera Khurd, Delhi

Sample collection kit: sterile 250 mL containers, ethanol and a PPE kit carried in a basket

Sample collection from the STP at Kakroi, receives wastewater from Sector 23, Sonipat, 25 MLD plant capacity

Ashoka university

Najafgarh, Delhi

Prof Anurag Agrawal addressing the global impact of antimicrobial resistance (AMR) and advocating for an integrated one health approach for combatting

Capturing the camaraderie and shared learning of the workshop with a group photo featuring all participants gathered together

Prof. Anurag Agrawal and Prof Shashidhara participating in discussions

Prof Shashidhara detailing the RF-APSI, efforts in AMR surveillance.

Dr. Anuj Sharma from WHO highlighting the importance of AMR and environmental surveillance, highlighting the importance of data-driven action.

Lunch time networking

Dr. Rakesh Mishra speaking about the importance of low cost kits in surveillance

Partners of RF-APSI listening to the talks

Panel discussion on Data Management Systems for AMR Surveillance Data, Importance of Standardized Reporting Formats and Protocols, Utilizing Surveillance Data for Decision-making and Policy Development.

Medical students of GIMS Greater Nodia at the AMR Frontline Workshop in Delhi.

Students taking the oath for responsible use of antibiotics, stringent infection control practices and AMR awareness.

Medical students engaging in an interactive game called Taboo during the workshop.

Ms Sehr Brar (AMR Team, WHO India) engaging with medical students on national and state action plans for AMR.

Dr. Anuj Sharma (AMR Team, WHO India) engaging with medical students at UCMS, Delhi.

Medical students of GIMS Greater Nodia at the AMR Frontline Workshop in Delhi.

Dr. Shraddha Karve (Faculty Fellow, Ashoka University) introducing APSI-India’s surveillance initiatives.

Dr. Sonal Saxena (Director Professor and HOD, Microbiology, MAMC) shedding light on infection control practices to curb AMR.

Dr. Hitakshi Sharma, Dr. Sonal Saxena and Dr. Bansidhar Tarai in the panel discussion “Doctors Decode AMR.”

Dr. Bansidhar Tarai (Associate Director and HOD, Molecular Diagnostics & Infection Control Departments, Max Hospitals) talking about the digital management of the AMR Stewardship Program at Max Hospitals.

Healthcare Professionals of GIMS Greater Nodia at the AMR Frontline Workshop in Delhi.

Participants of Point-of-care Nanopore based sequencing workshop for AMR surveillance performing DNA extraction

Participants of Point-of-care Nanopore based sequencing workshop for AMR surveillance performing DNA extraction

Participants of Point-of-care Nanopore based sequencing workshop for AMR surveillance performing Library prep for sequencing

Sequencing analysis training module

Participants with instructors Dr. Rajesh Pandey(CSIR IGIB) & Dr. Shraddha Karve(Ashoka University)

Run through the sequencing module with the attendees of workshop

APSI-India at World Health Summit Regional Meeting (April 26, 2025)